Technical Documentation

Information about documentation standards and analytical methods for research peptides.

Documentation Overview

Peptide Hackers provides comprehensive technical documentation to support research quality and reproducibility. Each product batch is accompanied by analytical data generated using validated laboratory methods.

Certificate of Analysis

Each production lot receives a Certificate of Analysis (COA) documenting analytical test results. COAs include:

  • Product identification and batch number
  • Analysis date and testing laboratory reference
  • Purity determination by HPLC
  • Mass spectrometry confirmation
  • Additional quality parameters as applicable
  • Specification limits and actual results

COAs are batch-specific documents. Results apply only to the specific lot referenced on the certificate.

Analytical Methods

High-Performance Liquid Chromatography (HPLC)

Purity is determined by reversed-phase HPLC with UV detection at 214-220 nm. Peak area integration provides quantitative purity expressed as percentage of total peak area.

Mass Spectrometry

Molecular identity is confirmed by mass spectrometry (ESI-MS or MALDI-TOF). Observed molecular mass is compared against theoretical mass calculated from the amino acid sequence.

Amino Acid Analysis

Where specified, amino acid composition is verified by acid hydrolysis followed by chromatographic amino acid quantitation.

Water Content

Moisture content is determined by Karl Fischer titration. Results are reported as percentage of total mass.

Endotoxin Testing

Bacterial endotoxin levels are assessed by Limulus amebocyte lysate (LAL) assay where specified. Results are reported in endotoxin units per milligram.

Specifications

Product specifications represent acceptance criteria established based on analytical method capabilities and research-grade quality standards:

PurityMinimum ≥98% by HPLC
IdentityMass accuracy within specified tolerance
Peptide ContentMinimum ≥80%
Water ContentMaximum ≤8%
EndotoxinWithin specified limits where applicable

Documentation Access

Certificates of Analysis are accessible through the COA section of this website. Each COA is identified by a unique document reference corresponding to the product batch number.

View Certificates of Analysis

Documentation Purpose

Technical documentation is provided to support research quality including verification of material identity, laboratory record-keeping, research publication methodology, and institutional compliance requirements.

Documentation is not intended for regulatory submission and does not constitute certification for pharmaceutical or diagnostic applications.