TB-500 (Thymosin Beta-4) Research Peptide: Laboratory Studies in Cellular Migration and Angiogenesis

Introduction to TB-500: A Synthetic Thymosin Beta-4 Fragment for Research

TB-500 is a synthetic version of the naturally occurring Thymosin Beta-4 peptide fragment, consisting of 43 amino acids with the molecular formula C212H350N56O78S. This research compound is extensively studied in laboratory settings for its role in actin sequestration, cellular migration pathways, and angiogenesis mechanisms. As a G-actin binding protein, TB-500 serves as a valuable research tool for investigating cytoskeletal dynamics and cellular motility in controlled experimental conditions.

Research Mechanisms: Molecular Pathways and Cellular Interactions

Actin Regulation Studies

Laboratory investigations demonstrate TB-500's role in actin polymerization and depolymerization cycles. Research shows this peptide's ability to sequester G-actin monomers, affecting cytoskeletal reorganization in various cell culture models. Studies focus on understanding cellular morphology changes and migration patterns in vitro.

Angiogenesis Research Applications

Cell culture experiments reveal TB-500's effects on endothelial cell behavior and vascular tube formation. Research models demonstrate altered VEGF expression patterns and endothelial cell proliferation rates under controlled laboratory conditions. Studies examine capillary-like structure formation in matrigel assays.

Cell Migration and Motility Studies

In vitro scratch assays and transwell migration experiments show modified cellular movement patterns in the presence of TB-500. Research focuses on lamellipodial extension, focal adhesion dynamics, and directional cell migration in various cell lines including fibroblasts and keratinocytes.

Anti-Inflammatory Pathway Research

Laboratory studies investigate TB-500's modulation of inflammatory signaling cascades. Research demonstrates altered cytokine production profiles and NF-κB pathway activity in immune cell models. Experiments examine prostaglandin synthesis and inflammatory mediator expression.

Laboratory Applications and Experimental Protocols

Cell Culture Research Parameters

Standard research protocols utilize TB-500 concentrations ranging from 0.1-100 μM in cell culture studies. Experimental designs include: - Proliferation assays using various cell lines - Migration studies in wound scratch models - Differentiation experiments in stem cell cultures - Apoptosis resistance studies in stress conditions

Animal Model Research Protocols

Laboratory animal studies typically employ doses of 2-5 mg/kg in experimental designs. Research applications focus on: - Tissue response mechanisms in controlled injury models - Molecular pathway activation studies - Biomarker expression analysis - Pharmacokinetic and biodistribution studies

Molecular Biology Research Tools

TB-500 serves as a research reagent for: - Gene expression profiling studies - Protein-protein interaction assays - Signal transduction pathway analysis - Cytoskeletal dynamics investigations

Research Storage and Handling Protocols

Lyophilized TB-500 should be stored at -20°C or below for long-term stability. Reconstituted solutions in bacteriostatic water or appropriate research buffers should be stored at 2-8°C and used within 30 days. Avoid repeated freeze-thaw cycles. Follow standard peptide handling procedures and institutional biosafety guidelines.

Quality Specifications for Research Use

Research-grade TB-500 specifications: - Purity: ≥98% by HPLC - Molecular Weight: 4963.5 Da - Appearance: White lyophilized powder - Solubility: Readily soluble in water and aqueous buffers - Endotoxin: <1 EU/mg (LAL method)

Important Research Notice

This material is provided exclusively for in vitro research and laboratory animal studies. It is not intended, approved, or suitable for human or veterinary therapeutic use. Researchers must comply with all applicable regulations and institutional review board requirements. This product is available only to qualified research institutions and investigators conducting legitimate scientific research.

Certificate of Analysis Available

Each batch includes comprehensive analytical documentation including HPLC chromatograms, mass spectrometry data, and purity analysis. Research institutions can request batch-specific certificates for quality assurance purposes.