Peptide Protocol Database - Research Reference Guide

Please be advised: The Peptide Protocol Database and the Authors of the Protocol Database cannot be held responsible for your own medical and health decisions or any damage or harm caused by the use of this information. All of these peptide protocols and doses are for educational and research use only. All the products we offer are intended for laboratory research use only and are not approved for human consumption. These substances are unsuitable for human consumption, in clinical or any other unsupervised environment.

What Are Peptides?

Peptides are short amino acid chains (2–50 amino acids in length) that act as messengers in biological organisms. Synthetic peptides are used under lab conditions to study certain cellular pathways, receptor interactions, and physiological mechanisms.

Research Applications

In experimental settings, peptides are used to study:

  • Signaling and receptor ligand binding in the cell
  • Metabolic processes and energy regulation
  • Tissue repair and regeneration mechanisms
  • Immune system responses and modulation
  • Neurotransmitter function and cognitive pathways

Categories of Research Peptides

Growth Hormone Secretagogues

Peptides such as CJC-1295, Ipamorelin, and GHRP-6 are examined for their ability to stimulate growth hormone release in laboratory animals.

Metabolic Research Compounds

GLP-1 agonists (Semaglutide, Tirzepatide) and lipolytic peptides (AOD-9604) are investigated for metabolic pathway research.

Tissue Repair Peptides

BPC-157, TB-500 and GHK-Cu are reviewed for their involvement in the processes of cellular repair and regeneration.

Longevity Research Compounds

Epitalon, NAD+ and FOXO4-DRI are the subjects of aging research and cellular senescence models.

Neuropeptides

Semax, Selank, and Cerebrolysin are also being used as research chemicals in neuroscience to study various effects such as cognitive function and neuroprotection.

Safety & Handling for the Laboratory

Storage Requirements

  • Freeze-dried peptides: For storage at -20°C or lower
  • Reconstituted peptides: Refrigerate at 2-8°C
  • Protect from light and moisture
  • Use within a stability window (commonly 2-4 weeks post-reconstitution)

Reconstitution Guidelines

  1. Reconstitute the lyophilized powder with the solvents that is recommended for use of the material (sterile water, 0.9% saline solution, or the bacteriostatic water (0.9% benzyl alcohol)), acetic acid or DMSO
  2. Concentration determined by experimental needs
  3. Sterilise by filtration, if necessary, for cell culture applications
  4. Aliquot to avoid freeze-thaw cycles

Laboratory Safety Protocols

  • Wear PPE (gloves, lab coat, safety glasses)
  • Work in well ventilated or biosafety cabinets
  • Adhere to the chemical handling standards of the institution
  • Dispose of in accordance with regulations

Common Research Protocols (Reference Only)

The following information is extracted from published research literature to be used as reference material. These are not guidelines or usage instructions.

Metabolic Research Protocols

Semaglutide studies: As a research tool, the GLP-1 pathway activation and metabolic responses are usually evaluated based on weekly 0.25–2.4mg dosing regimen.

AOD-9604 study: Lab studies use 250-500mcg per day to investigate lipolytic mechanisms without growth hormone stimulation.

Tissue Repair Studies

BPC-157 dosing protocols: Investigative experiments utilise 250-500mcg of BPC157 each day in injury and recovery studies.

TB-500 studies: Loading phases of 2-5mg twice per week are shown in tissue repair research to be followed by maintenance programs.

Longevity Research

Epitalon research: Research on telomerase in the range of 5-10mg daily for 10 days followed by 10 days off for 2-3 cycles.

NAD+ protocols: 50 - 100mg are used for metabolic research on cell energy production and mitochondrial function.

Research Perspective: FAQ

Q: Where can scientists find high-quality peptides?

A: Only buy from suppliers providing certificate of analysis, operating under the research chemical regulations, and with a visible QC/testing policy.

Q: What grades of purity are called for in research?

A: All research applications are different, but most require a min of 95% purity peptides. Higher purities (>98%) may be required for some sensitive applications.

Q: How do I document research data?

A: Document your experiments including lot numbers, concentrations and experimental conditions and observations. Obey to good laboratory practice (GLP) rules.

Q: Can these peptides now be tested in human trials?

A: Human studies need approval (IND with FDA) and institutional review board oversight. The peptides in this database are intended for the preclinical studies only.

Q: What kind of research is this used for?

A: Typical applications are cell culture, animal research, receptor binding studies and mechanistic research. Any use of the work other than as authorized under this license or copyright law is prohibited.

Final Notice - Research Use Only

This database and other information therein is provided for educational and research purposes only.

You agree that any information you would have obtained by using this tool is not advice or a substitute for advice from an attorney.

  • All of the compounds mentioned here are intended for research purposes
  • Nothing here is medical advice or human use advice
  • You will abide by all applicable local, state, national and international laws and regulations
  • You are aware they are not for human consumption or any sort of therapeutic use

Abuse of research chemicals can lead to severe legal and health issues. And remember to have proper supervision and obey all safety standards.